Spanish version: Toma de muestras de agua

Requirements To Collect Water Samples For Chemical and Microbiological Analyses

Parameter	Type of container	Minimum quantity of sample	Preservation	Maximum permissible storage time
Turbidity	P or V	100 ml	refrigerate, 10°C	48 hours
Alkalinity	P or V	50 ml	refrigerate, 4°C	48 hours
Chlorine residual	P or V	500 ml	analyze immediately
Colour	P or V	500 ml	refrigerate, 4°C	48 hours
Conductivity	P or V	500 ml	refrigerate, 4°C	28 days
Hardness	P or V	100 ml	add HNO3up to pH < 2	6 months
Solids	P or V	1000ml	refrigerate, 4°C	2-7 days
Settleable solids	.	.	.	.
Chloride	P or V	100 ml	refrigerate, 4°C	7 days
Fluorides	P	10 ml	refrigerate, 4°C	7 days
Sulfates	P or V	100 ml	refrigerate, 4°C	25 days
Cyanides	P or V	500 ml	refrigerate, add NaOH up to pH = 12	14 days, 24 hours in sulfides presence
Oils and greases	V	1000 ml	refrigerate, add HCl up to pH < 2	.
Chlorine demand	P or V	3000 ml	refrigerate, 4°C	.
Hydrocarbons	P or V	1000 ml	refrigerate, add HCl up to pH < 2	7 days
Nitrogen	P or V	250 ml	refrigerate, add H2SO4 up to pH < 2	23 days
Ammoniacal nitrogen	P or V	50 ml	refrigerate, add H2SO4 up to pH < 2	24 hours
Organic nitrogen	P or V	250 ml	refrigerate, add H2SO4 up to pH < 2	28 days
Nitrates	P or V	100 ml	refrigerate, 4°C	28 days
Nitrites	P or V	100 ml	refrigerate, 4°C	48 hours
Total phosphorus	P or V	100 ml	refrigerate, 4°C	24 hours
Soluble phosphorus	P or V	100 ml	refrigerate, 4°C	24 hours
Phosphorus hidrolizable	P or V	10 ml	refrigerate, 4°C	24 hours
Dissolved oxygen	V	300 ml	analyze immediately	30 min.
BOD	P or V	1000 ml	refrigerate, 4°C	24 hours
COD	P or V	10 ml	refrigerate, add H2SO4 up to pH < 2	28 days
SAAM	P or V	100 ml	refrigerate, 4°C	24 hours
Metals	P or V	100 ml	add HNO3up to pH < 2	6 months
Arsenic	P or V	50 ml	refrigerate, add HNO3up to pH < 2	6 months
Mercury	V	100 ml	refrigerate, add H2SO4up to pH < 2	28 days
Hetherotrophic bacteria	* V/P	200 ml	refrigerate, 4°C	24 hours
Total coliforms (MPN)	V/P	200 ml	refrigerate, 4°C	24 hours
Total coliforms (FM)	V/P	200 ml	refrigerate, 4°C	24 hours
Fecal coliforms (MPN)	V	200 ml	refrigerate, 4°C	24 hours
Fecal coliforms (FM)	V	200 ml	refrigerate, 4°C	24 hours
Salmonella (A/P)	V	200 ml	refrigerate, 4°C	24 hours
Salmonella (MPN)	V	200 ml	refrigerate, 4°C	24 hours
Escherichia (A/P)	V	200 ml	refrigerate, 4°C	24 hours
Escherichia (MPN)	V	200 ml	refrigerate, 4°C	24 hours
Sulfate clostridium	V	200 ml	refrigerate, 4°C	24 hours
Fecal streptococcus	V	200 ml	refrigerate, 4°C	24 hours
Vibrio cholerae A/P	V	200 ml	refrigerate, 4°C	24 hours
Vibrio cholerae MPN	V	200 ml	refrigerate, 4°C	24 hours
Chlorophyll	.	.	refrigerate, 4°C	24 hours
Enteroparatites, Raw wastewater	P	1 l	Ice refrigeration	24 hours
Treated wastewater	P	5 l	Ice refrigeration	24 hours
River water	P	5 l	Ice refrigeration	24 hours
Drinking water	P	10 l	Ice refrigeration	24 hours
Sludge	B	200 g	Ice refrigeration	3 days
Phytoplankton ** Eutrophic waters	P	1 l	Room temperature	24 hours
Olygotrophic waters	P	6 l	Room temperature	24 hours
Zooplankton Eutrophic waters	P	1 l	Room temperature	24 hours
Oligotrophic waters	P	6 l	Room temperature	24 hours

P = plastic
V = glass
B = plastic bag sealed

* Sterile
** Can be reserved with formalin, 40 ml of formalin tamponada (20 g of sodium borate plus 1 liter of formaldehide to 37%) for a liter of sample